Gene gun delivery of mRNA in situ results in efficient transgene expression and genetic immunization.

@article{Qiu1996GeneGD,
  title={Gene gun delivery of mRNA in situ results in efficient transgene expression and genetic immunization.},
  author={P Qiu and P R Ziegelhoffer and Jing Sun and Ning Sun Yang},
  journal={Gene therapy},
  year={1996},
  volume={3 3},
  pages={262-8}
}
The use of mRNA to transfer genetic information into mammalian somatic cells in vivo or ex vivo may be advantageous in a number of gene therapy protocols. Success in utilizing in vivo RNA delivery for transgene expression has been extremely limited, partially due to RNA instability and to the lack of an efficient intracellular delivery mechanism applicable to a wide variety of tissue or organ systems. We report here that a particle-mediated gene delivery technology can be used to effectively… CONTINUE READING