Gene gun bombardment-mediated expression and translocation of EGFP-tagged GLUT4 in skeletal muscle fibres in vivo

@article{Lauritzen2002GeneGB,
  title={Gene gun bombardment-mediated expression and translocation of EGFP-tagged GLUT4 in skeletal muscle fibres in vivo},
  author={Hans P. M. M. Lauritzen and Christine Reynet and Peter Schjerling and Evelyn Ralston and Steve Thomas and Henrik Galbo and Thorkil Ploug},
  journal={Pfl{\"u}gers Archiv},
  year={2002},
  volume={444},
  pages={710-721}
}
Cellular protein trafficking has been studied to date only in vitro or with techniques that are invasive and have a low time resolution. To establish a gentle method for analysis of glucose transporter-4 (GLUT4) trafficking in vivo in fully differentiated rat skeletal muscle fibres we combined the enhanced green fluorescent protein (EGFP) labelling technique with physical transfection methods in vivo: intramuscular plasmid injection or gene gun bombardment. During optimisation experiments with… CONTINUE READING

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