Gene-expression programs in embryogenic and non-embryogenic carrot cultures

@article{Wilde1988GeneexpressionPI,
  title={Gene-expression programs in embryogenic and non-embryogenic carrot cultures},
  author={H. Dayton Wilde and William S. Nelson and Hilbert Booij and Sacco C. de Vries and Terry L. Thomas},
  journal={Planta},
  year={1988},
  volume={176},
  pages={205-211}
}
Somatic embryogenesis can be synchronized by enriching carrot (Daucus carota L.) suspension cultures for small, dense clusters of cells termed proembryogenic masses (PEMs). Gene-expression programs of PEMs were compared with those of embryonic and mature tissues by in-vitro translation of representative mRNA populations and by nucleic-acid hybridization. Analysis of invitro-translated polypeptides by two-dimensional polyacrylamide gel electrophoresis revealed striking similarities between the… CONTINUE READING

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