Gene expression profiling in mouse liver infected with Clonorchis sinensis metacercariae

Abstract

Clonorchis sinensis, the parasite that causes clonorchiasis, is endemic in many Asian countries, and infection with the organism drives changes in the liver tissues of the host. However, information regarding the molecular events in clonorchiasis remains limited, and little is currently known about host–pathogen interactions in clonorchiasis. In this study, we assessed the gene expression profiles in mice livers via DNA microarray analysis 1, 2, 4, and 6 weeks after induced metacercariae infection. Functional clustering of the gene expression profile showed that the immunity-involved genes were induced in the livers of the mice at the early stage of metacercariae infection, whereas immune responses were reduced in the 6-week liver tissues after infection in which the metacercariae became adult flukes. Many genes involved in fatty acid metabolism, including Peci, Cyp4a10, Acat1, Ehhadh, Gcdh, and Cyp2 family were downregulated in the infected livers. On the other hand, the liver tissues infected with the parasite expressed Wnt signaling molecules such as Wnt7b, Fzd6, and Pdgfrb and cell cycle-regulating genes including cyclin-D1, Cdca3, and Bcl3. These investigations constitute an excellent starting point for increased understanding of the molecular mechanisms underlying host–pathogen interaction during the development of C. sinensis in the host liver.

DOI: 10.1007/s00436-009-1662-z

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@article{Kim2009GeneEP, title={Gene expression profiling in mouse liver infected with Clonorchis sinensis metacercariae}, author={Dong Min Kim and Byung-Sam Ko and Jung-Won Ju and Shin-Hyeong Cho and Suk-Jin Yang and Young Il Yeom and Tong-soo Kim and Yonggwan Won and Il-Chul Kim}, journal={Parasitology Research}, year={2009}, volume={106}, pages={269-278} }