Gene expression following transfection of fish cells.

  title={Gene expression following transfection of fish cells.},
  author={Monique B{\'e}arzotti and Elizabeth Gavignet Perrot and Christine Michard-Vanh{\'e}e and Genevi{\`e}ve Jolivet and J. Attal and M C Th{\'e}ron and Claudine Puissant and Michel Dreano and John J. Kopchick and Robert Powell},
  journal={Journal of biotechnology},
  volume={26 2-3},
Various genes containing different transcriptional regulatory elements (TRE) and the bacterial marker gene coding for chloramphenicol acetyl transferase were transfected into several fish cell lines to evaluate the efficiency of expression in comparison with mammalian cells. The CMV and RSV TRE were the most efficient non-inducible promoters in directing reporter gene expression. RSV and CMV appeared of similar potency in a stable fish cell line. The human HSP-70 promoter showed high potency in… CONTINUE READING
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