• DOI:10.1152/PHYSIOLGENOMICS.2001.6.3.169
• Corpus ID: 9296949

Gene expression following acute morphine administration.

@article{Loguinov2001GeneEF,
  title={Gene expression following acute morphine administration.},
  author={Alexander V. Loguinov and Leonard M. Anderson and Gregory J. Crosby and Rustam Yu. Yukhananov},
  journal={Physiological genomics},
  year={2001},
  volume={6 3},
  pages={
          169-81
        }
}

• A. Loguinov, L. Anderson, +1 author R. Yukhananov
• Published 28 August 2001
• Biology
• Physiological genomics

The long-term response to neurotropic drugs depends on drug-induced neuroplasticity and underlying changes in gene expression. However, alterations in neuronal gene expression can be observed even following single injection. To investigate the extent of these changes, gene expression in the medial striatum and lumbar part of the spinal cord was monitored by cDNA microarray following single injection of morphine. Using robust and resistant linear regression (MM-estimator) with simultaneous prediction confidence intervals, we detected differentially expressed genes. By combining the results with cluster analysis, we have found that a single morphine injection alters expression of two major groups of genes, for proteins involved in mitochondrial respiration and for cytoskeleton-related proteins. RNAs for these proteins were mostly downregulated both in the medial striatum and in lumbar part of the spinal cord. These transitory changes were prevented by coadministration of the opioid antagonist naloxone. Data indicate that microarray analysis by itself is useful in describing the effect of well-known substances on the nervous system and provides sufficient information to propose a potentially novel pathway mediating its activity.