Gene expression analysis of plant host-pathogen interactions by SuperSAGE.

  title={Gene expression analysis of plant host-pathogen interactions by SuperSAGE.},
  author={Hideo Matsumura and Stefanie Reich and Akiko Ito and Hiromasa Saitoh and Sophien Kamoun and Peter Winter and Gunter Kahl and Monika Reuter and Detlev H Kruger and Ryohei Terauchi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={100 26},
The type III restriction endonuclease EcoP15I was used in isolating fragments of 26 bp from defined positions of cDNAs. We call this substantially improved variant to the conventional serial analysis of gene expression (SAGE) procedure "SuperSAGE." By applying SuperSAGE to Magnaporthe grisea (blast)-infected rice leaves, gene expression profiles of both the rice host and blast fungus were simultaneously monitored by making use of the fully sequenced genomes of both organisms, revealing that the… CONTINUE READING


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