Gene expression analysis of calcineurin isoforms in T-lymphocytes--a method applied on kidney-transplant recipients.

@article{Mortensen2010GeneEA,
  title={Gene expression analysis of calcineurin isoforms in T-lymphocytes--a method applied on kidney-transplant recipients.},
  author={D. M. Mortensen and R. Roge and A. {\O}zbay and P. Koefoed-Nielsen and K. J{\o}rgensen},
  journal={Transplant immunology},
  year={2010},
  volume={23 1-2},
  pages={
          24-7
        }
}
  • D. M. Mortensen, R. Roge, +2 authors K. Jørgensen
  • Published 2010
  • Biology, Medicine
  • Transplant immunology
  • BACKGROUND Tacrolimus exerts its immunosuppressive effect through inhibition of the intracellular enzyme calcineurin phosphatase (CaN). In this study, we set-up a validated real-time PCR method to measure the gene expression of the two major isoforms of the catalytic subunit of CaN in T-lymphocytes. METHODS 20 stable kidney-transplant recipients, 10 early kidney-transplant recipients and 10 healthy non-medicated subjects had blood drawn and T-lymphocytes were isolated using E-rosette gradient… CONTINUE READING
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