Gene expression analysis in colorectal cancer using practical DNA array filter.

@article{Okuno2001GeneEA,
  title={Gene expression analysis in colorectal cancer using practical DNA array filter.},
  author={Kiyotaka Okuno and Masayuki Yasutomi and Noriko Nishimura and Toshiya Arakawa and Mikio Shiomi and Jin‐ichi Hida and Kazuhiro Ueda and Kahori Minami},
  journal={Diseases of the colon and rectum},
  year={2001},
  volume={44 2},
  pages={295-9}
}
PURPOSE We examined the usability of a newly developed, compact-sized DNA array filter for studying the gene expression pattern of individual colorectal cancer. METHODS Complementary DNA probes were prepared from mRNA extracted from colonic cancer specimens and adjacent normal mucosa and then were labeled with chemiluminescence. These labeled probes were allowed to bind to the gene fragments on the filter. A specialized scanning charge-coupled device camera measured the intensity of each… CONTINUE READING

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