Gene expression analysis for high throughput screening applications.

  title={Gene expression analysis for high throughput screening applications.},
  author={Albert Pinhasov and Jay Mei and Dhammika Amaratunga and Frank A. Amato and Hong Lu and Jack A Kauffman and Hong Xin and Douglas E. Brenneman and Dana L. Johnson and Patricia Andrade-Gordon and Sergey E. Ilyin},
  journal={Combinatorial chemistry & high throughput screening},
  volume={7 2},
To meet growing needs for high throughput gene expression profiling, we established a new automated high throughput TaqMan RT-PCR method for quantitative mRNA expression analysis. In this method, the Allegro( trade mark ) (Zymark) system conducts all sample tracking and liquid handling steps, and ABI PRISM 7900 HT (Applied Biosystems) is used to conduct real-time determination of the C(t) value when amplification of PCR products is first detected and accumulation of inhibitory PCR products is… CONTINUE READING