Gene expression analysis by real-time PCR: experimental demonstration of PCR detection limits.

Abstract

Reverse transcription followed by real-time PCR (RT-qPCR) is the gold standard for quantifying gene expression. However, because of PCR detection limits, theorized to be three template copies, the quantification of genes exhibiting great expression variability is challenging. Using genes with high to low expression in rat tissues we experimentally… (More)
DOI: 10.1016/j.ab.2012.09.029

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