Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays

  title={Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays},
  author={Sydney Brenner and Maria Q. Johnson and John A. Bridgham and George Golda and David H. Lloyd and David A. Johnson and Shujun Luo and Sarah N. Mccurdy and M Foy and Mark Ewan and Rithy K. Roth and Dave George and Sam Eletr and Glenn Albrecht and Eric H. Vermaas and Steven R. Williams and Keith Moon and Timothy S. Burcham and Michael Pallas and Robert B. Dubridge and James Kirchner and Karen L. Fearon and Jen-i Mao and Kevin Corcoran},
  journal={Nature Biotechnology},
We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 μm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing microbeads in a flow cell at a density greater than 3 × 106 microbeads/cm2. Sequences of the free ends of the cloned templates on each microbead were then simultaneously analyzed using a… 

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