Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays

  title={Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays},
  author={S. Brenner and M. Johnson and John A. Bridgham and George Golda and David H. Lloyd and D. Johnson and S. Luo and S. Mccurdy and M. Foy and Mark Ewan and Rithy K. Roth and D. George and S. el-Etr and Glenn Albrecht and E. Vermaas and Steven R. Williams and K. Moon and Timothy S. Burcham and M. Pallas and Robert B. DuBridge and J. Kirchner and K. Fearon and J. Mao and K. Corcoran},
  journal={Nature Biotechnology},
  • S. Brenner, M. Johnson, +21 authors K. Corcoran
  • Published 2000
  • Medicine, Biology
  • Nature Biotechnology
  • We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 μm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing microbeads in a flow cell at a density greater than 3 × 106 microbeads/cm2. Sequences of the free ends of the cloned templates on each microbead were then simultaneously analyzed using a… CONTINUE READING
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