Gene expression: RNA interference in adult mice

@article{McCaffrey2002GeneER,
  title={Gene expression: RNA interference in adult mice},
  author={Anton P. McCaffrey and Leonard Meuse and Thu-Thao T. Pham and Douglas S. Conklin and Gregory J. Hannon and Mark A. Kay},
  journal={Nature},
  year={2002},
  volume={418},
  pages={38-39}
}
RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo. 
RNA‐Interference‐Based Silencing of Mammalian Gene Expression
  • D. Conklin
  • Biology, Medicine
  • Chembiochem : a European journal of chemical biology
  • 2003
TLDR
The cellular expression from DNA vectors of shRNAs, which are processed by the Dicer enzyme into si RNAs, allows the creation of continuous cell lines and transgenic animals in which suppression of a target gene is stably maintained by RNAi. Expand
RNA interference of HIV replication.
TLDR
Double-stranded RNA-mediated interference induces sequence-specific post-transcriptional gene silencing and has emerged as a powerful tool to silence gene expression in multiple organisms, opening a new avenue for gene-based therapeutics. Expand
RNA interference gene therapy: RNA interference gets infectious
TLDR
Two manuscripts in Nature Genetics demonstrate that RNAi delivered by retroviral and lentiviral vectors can silence genes in mice by demonstrating the promise of RNAi gene therapy using viral vectors. Expand
Using RNA interference to modulate gene expression
TLDR
Various strategies currently being developed to employ RNAi technology for rapid functional genomic analyses and therapeutic applications will be reviewed in this paper. Expand
RNA interference in mammalian cell systems.
  • P. Paddison
  • Biology, Medicine
  • Current topics in microbiology and immunology
  • 2008
TLDR
The use of RNA interference to evoke gene silencing in mammalian cells has almost become routine laboratory practice and the first genome-wide loss of function screens have been carried out in mammals. Expand
RNA silencing in viral infections: insights from poliovirus.
TLDR
The current data and perspectives on the use of RNA interference in the study of poliovirus as a model for positive strand RNA viruses are reviewed and discussed. Expand
[Gene therapy of virus replication with RNAi].
TLDR
The short interference RNA (siRNA) cleaves target RNA even in mammalian cells without adverse effects of long dsRNA such as an interferon response, and works much more efficiently than antisense oligonucleotide and ribozyme. Expand
Targeting genes in living mammals by RNA interference.
TLDR
RNAi-based tools for silencing genes in living mammals include: (i) transgenic RNAi strategies, where RNAi is triggered by a transgene transmitted through the germline and (ii) approaches, where an RNAi trigger is delivered into an adult animal. Expand
Study of hepatocytes using RNA interference
TLDR
Various factors involved in the regulation of hepatocyte function have been identified using RNAi, indicating that RNAi is a useful strategy for characterization and a promising new therapeutic strategy. Expand
RNA interference: gene silencing in the fast lane.
TLDR
Use of RNAi in cancer research and as a possible therapeutic tool for cancer treatment are discussed. Expand
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