Gene enrichment in maize with hypomethylated partial restriction (HMPR) libraries.

Abstract

A new technology was developed to assist gene-enrichment sequencing of any complex plant genome, employing maize as the test organism. Hypomethylated partial restriction (HMPR) libraries were constructed by using independent partial digestions with methylation-sensitive restriction enzymes HpaII (5'-CCGG-3') and HpyCH4IV (5'-ACGT-3'). Fragments of 1-4 kb… (More)

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