Gene-editing nucleases

@article{Baker2011GeneeditingN,
  title={Gene-editing nucleases},
  author={Monya Baker},
  journal={Nature Methods},
  year={2011},
  volume={9},
  pages={23-26}
}
  • M. Baker
  • Published 28 December 2011
  • Biology
  • Nature Methods
ways to modify sequences in the genome, a powerful technique. Even as the genome sequences of many organisms are being revealed, understanding what those genes really do requires tweaking the sequences, deleting or modifying genes, and studying the consequences. With the exceptions of those from mice and yeast, however, eukaryotic genomes generally resist this type of precise tinkering. Engineered gene-editing nucleases are now poised to change that. Over the past 15 years, researchers have… 

Applications of genome editing tools in drug discovery and basic research

This work developed an interface between genome editing and proteomics to isolate native protein complexes produced from their natural genomic contexts and uncovered subunits and interactions among well-characterized complexes and report the isolation of novel Mettl3-binding partners.

Genome Editing for Crop Improvement: Status and Prospects

This chapter reviews the CRISPR-Cas strategy by focusing on components of the tool kit and available variants, delivery into plant cells, and gene modification detection assays.

TALENs: customizable molecular DNA scissors for genome engineering of plants.

Genome Editing in Cancer Research and Cure

Current applications of cancer research and cure, and the possibility of genome editing being the possible cause of enhancing the risk of cancer development, are discussed in this chapter.

Technology developments in biological tools for targeted genome surgery

This review presents a comprehensive and concise description of TALENs technology developments for targeted genome surgery with to the point description and comparison of other tools.

Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome

Results presented here indicate the TALENs are highly sequence-specific and produce minimal off-target effects.

Targeted Gene Mutation in Plants

Three powerful techniques, including zinc-finger nucleases, transcription activator-like effector nucleases (TALENs), and RNA-guided endonucleases (RGENs), have been developed for targeted DNA sequence modifications.

Engineering Designer Transcription Activator‐‐Like Effector Nucleases (TALENs) by REAL or REAL‐Fast Assembly

Detailed protocols for the serial, hierarchical assembly of TALENs that require neither PCR nor specialized multi‐fragment ligations and that can be implemented by any laboratory are described.

CRISPR/Cas9: A Revolutionary Tool for Recent Advances in Crop Improvement: A Review

The current applications of genome editing in plants, focuses on its potential for crop improvement in terms of adaptation, resilience, and end-users' needs.
...