Gene editing: not just for translation anymore

@article{McMahon2011GeneEN,
  title={Gene editing: not just for translation anymore},
  author={Moira A. McMahon and Meghdad Rahdar and Matthew H. Porteus},
  journal={Nature Methods},
  year={2011},
  volume={9},
  pages={28-31}
}
Engineered nucleases have advanced the field of gene therapy with the promise of targeted genome modification as a treatment for human diseases. Here we discuss why engineered nucleases are an exciting research tool for gene editing and consider their applications to a range of biological questions. 

Special Issue: Manifesting Synthetic Biology

TLDR
The existing assays for quantifying on- and off-target genome editing outcomes are reviewed and their utility in advancing the technology is described and their importance for the genome editing field is discussed.

Quantifying on- and off-target genome editing.

Key to Delivery: The (Epi-)genome Editing Vector Toolbox.

TLDR
Current viral and non-viral delivery approaches applicable for genome and epigenome editing are summarized and discussed and their respective advantages and limitations are discussed.

Improvements in Gene Editing Technology Boost Its Applications in Livestock

TLDR
The methods developed to improve efficiency and specificity of gene editing tools as well as approaches that can be employed for gene regulation, base editing, and epigenetic modifications are discussed.

Assembly and characterization of megaTALs for hyperspecific genome engineering applications.

TLDR
This chapter describes the process of assembling a megaTAL from a meganuclease, as well as a method for characterization of nuclease cleavage activity in vivo using a fluorescence reporter assay.

Applications of CRISPR/Cas9 for Gene Editing in Hereditary Movement Disorders

TLDR
The applicability of CRISPR/Cas9 to preclinical studies or gene therapy in hereditary movement disorders is discussed and advantages in terms of clinical applicability over other genome editing technologies such as zinc-finger nucleases and transcription activator-like effector nucleases are discussed.

megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering

TLDR
The development of a single-chain rare-cleaving nuclease architecture is described, which is designated ‘megaTAL’, in which the DNA binding region of a transcription activator-like (TAL) effector is used to ‘address’ a site-specific meganuclease adjacent to a single desired genomic target site.

Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing.

Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells.

...

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TLDR
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TLDR
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