Gene dosage affects the expression of the duplicated NHP6 genes of Saccharomyces cerevisiae.

@article{Kolodrubetz2001GeneDA,
  title={Gene dosage affects the expression of the duplicated NHP6 genes of Saccharomyces cerevisiae.},
  author={David Kolodrubetz and M Kruppa and Alex Burgum},
  journal={Gene},
  year={2001},
  volume={272 1-2},
  pages={93-101}
}
Nhp6Ap and Nhp6Bp, which are 87% identical in sequence, are moderately abundant, chromosome-associated proteins from Saccharomyces cerevisiae. In wild type cells Nhp6Ap is present at three times the level of Nhp6Bp. The effects of altering NHP6A or NHP6B gene number on the expression of its partner has been examined using Northern blots and reporter genes. Deletion of NHP6A led to a three-fold increase in NHP6B synthesis while an extra copy of NHP6A reduced NHP6B expression two-fold. Changes in… CONTINUE READING

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