Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition

@article{Hu2005GeneDI,
  title={Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition},
  author={Guanggan Hu and James W Kronstad},
  journal={Current Genetics},
  year={2005},
  volume={49},
  pages={341-350}
}
Cryptococcus neoformans and Cryptococcus gattii are basidiomycetous fungi that infect immunocompromised and immunocompetent people. We developed an insertional mutagenesis strategy for these species based on in vitro transposition and we tested the method by disrupting the URA5 gene in a strain of C. neoformans and the CAP10 gene in three strains of C. gattii. We targeted plasmid DNA containing the URA5 gene or plasmid DNA containing the CAP10 gene from genomic libraries from the shotgun… CONTINUE READING

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