Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA.

@article{Ramakrishna2014GeneDB,
  title={Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA.},
  author={Suresh Ramakrishna and Abu-Bonsrah Kwaku Dad and Jagadish Beloor and Ramu Gopalappa and Sang-Kyung Lee and Hyongbum Kim},
  journal={Genome research},
  year={2014},
  volume={24 6},
  pages={1020-7}
}
RNA-guided endonucleases (RGENs) derived from the CRISPR/Cas system represent an efficient tool for genome editing. RGENs consist of two components: Cas9 protein and guide RNA. Plasmid-mediated delivery of these components into cells can result in uncontrolled integration of the plasmid sequence into the host genome, and unwanted immune responses and potential safety problems that can be caused by the bacterial sequences. Furthermore, this delivery method requires transfection tools. Here we… CONTINUE READING

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