Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium.

@article{Li2016GeneDB,
  title={Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium.},
  author={Cheng-Lin Frank Li and Balaji Srinivasan Santhanam and Amanda Nicole Webb and Blaz Zupan and Gad Shaulsky},
  journal={Genome research},
  year={2016},
  volume={26 9},
  pages={
          1268-76
        }
}
Whole-genome sequencing is a useful approach for identification of chemical-induced lesions, but previous applications involved tedious genetic mapping to pinpoint the causative mutations. We propose that saturation mutagenesis under low mutagenic loads, followed by whole-genome sequencing, should allow direct implication of genes by identifying multiple independent alleles of each relevant gene. We tested the hypothesis by performing three genetic screens with chemical mutagenesis in the… CONTINUE READING
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References

Publications referenced by this paper.
SHOWING 1-10 OF 43 REFERENCES

Mutagenesis and gene identification in Dictyostelium by shotgun antisense.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1996
VIEW 7 EXCERPTS
HIGHLY INFLUENTIAL

A comparison of mutation spectra detected by the Escherichia coli Lac reversion assay and the Salmonella typhimurium His reversion assay

T. Ohta
  • Mutagenesis 15: 317–323.
  • 2000
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

AlteredN-glycosylationmodulates TgrB1- and TgrC1-mediated development but not allorecognition in Dictyostelium

Li C-LF, ChenG, WebbAN, G Shaulsky
  • J Cell Sci
  • 2015

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