Gene delivery to muscle.

  title={Gene delivery to muscle.},
  author={Matthew L. Springer and Thomas A Rando and Helen M. Blau},
  journal={Current protocols in human genetics},
  volume={Chapter 13},
The delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or "knockout" mouse models. This unit describes the isolation and retroviral transduction of mouse myoblasts, the injection of myoblasts and plasmid DNA into mouse muscle, and histological methods for analyzing the recipient muscle. A procedure describing the injection… CONTINUE READING