Gene delivery to human bone marrow mesenchymal stem cells by microporation.

@article{Madeira2011GeneDT,
  title={Gene delivery to human bone marrow mesenchymal stem cells by microporation.},
  author={Catarina Madeira and Sofia C. Ribeiro and Irina S. M. Pinheiro and Sofia Aires M Martins and Pedro Z. Andrade and Cl{\'a}udia L da Silva and Joaquim M S Cabral},
  journal={Journal of biotechnology},
  year={2011},
  volume={151 1},
  pages={130-6}
}
Electroporation has been considered one of the most efficient non-viral based methods to deliver genes regardless of frequently observed high cell mortality. In this study we used a microporation technique to optimise the delivery of plasmid DNA encoding green fluorescence protein (GFP) to human bone marrow mesenchymal stem cells (BM-MSC). Using resuspension buffer (RB) and as low as 1.5 x 10⁵ cells and 1 μg of DNA, we achieved 40% of cells expressing the transgene, with cell recovery and cell… CONTINUE READING

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Microporation is a valuable transfection method for gene expression in human adipose tissue-derived stem cells.

Molecular therapy : the journal of the American Society of Gene Therapy • 2009

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