Gene cloning of murine α-fetoprotein gene and construction of its eukaryotic expression vector and expression in CHO cells

@article{Jilin2003GeneCO,
  title={Gene cloning of murine α-fetoprotein gene and construction of its eukaryotic expression vector and expression in CHO cells},
  author={Yi Jilin and Tian Geng},
  journal={Journal of Huazhong University of Science and Technology [Medical Sciences]},
  year={2003},
  volume={23},
  pages={392-395}
}
To clone the murine α-fetoprotein (AFP) gene, construct the eukaryotic expression vector of AFP and express in CHO cells, total RNA were extracted from Hepa 1–6 cells, and then the murine α-fetoprotein gene was amplified by RT-PCR and cloned into the eukaryotic expression vector pcDNA3. 1. The recombinant of vector was identified by restriction enzyme… CONTINUE READING