Gene cloning of immunogenic antigens overexpressed in pancreatic cancer.

@article{Nakatsura2001GeneCO,
  title={Gene cloning of immunogenic antigens overexpressed in pancreatic cancer.},
  author={Tetsuya Nakatsura and Satoru Senju and Kenji Yamada and Toko Jotsuka and Michio Ogawa and Yasuharu Nishimura},
  journal={Biochemical and biophysical research communications},
  year={2001},
  volume={281 4},
  pages={
          936-44
        }
}
The serological analysis of recombinant cDNA expression libraries (SEREX) by utilizing a library derived from a human pancreatic adenocarcinoma cell line and IgG antibodies from an allogeneic patient serum led to the identification of 18 genes: 13 of these were known genes, and 5 were unknown genes. In Northern and RT-PCR analyses, we found that the expression of mRNA of 14 genes was elevated in pancreatic cancer cell lines compared with the levels in normal pancreatic tissues. In addition, the… CONTINUE READING
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