Gene cloning and characterization of alpha-glucuronidase of Bacillus stearothermophilus no. 236.

@article{Choi2000GeneCA,
  title={Gene cloning and characterization of alpha-glucuronidase of Bacillus stearothermophilus no. 236.},
  author={I D Choi and H Y Kim and Yeon Jin Choi},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2000},
  volume={64 12},
  pages={2530-7}
}
The alpha-glucuronidase gene of Bacillus stearothermophilus No. 236 was cloned, sequenced, and expressed in Escherichia coli. The gene, designated aguA, encoded a 691-residue polypeptide with calculated molecular weight of 78,156 and pI of 5.34. The alpha-glucuronidase produced by a recombinant E. coli strain containing the aguA gene was purified to apparent homogeneity and characterized. The molecular weight of the alpha-glucuronidase was 77,000 by SDS-PAGE and 161,000 by gel filtration; the… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

Molecular cloning of fungal xylanases: an overview

Applied Microbiology and Biotechnology • 2009
View 1 Excerpt
Highly Influenced

Similar Papers

Loading similar papers…