Gene cloning and characterization of a thermostable organic-tolerant α-amylase from Bacillus subtilis DR8806.

@article{Emtenani2015GeneCA,
  title={Gene cloning and characterization of a thermostable organic-tolerant α-amylase from Bacillus subtilis DR8806.},
  author={Shamsi Emtenani and Ahmad Asoodeh and Shirin Emtenani},
  journal={International journal of biological macromolecules},
  year={2015},
  volume={72},
  pages={290-8}
}
The gene encoding an extracellular α-amylase from Bacillus subtilis DR8806 was cloned into pET28a(+) vector and expressed in Escherichia coli BL21 (DE3). The recombinant enzyme with molecular mass of 76 kDa exhibited optimal activity at pH 5.0 and 70 °C with high stability in pH and temperature ranges of 4.0-9.0 and 45-75 °C. The enzyme showed a half-life of 125 min at 70 °C. The α-amylase activity enhanced in the presence of Na(+), K(+), and Ca(2+) ions, while Zn(2+), Pb(2+), and Hg(2+) ions… CONTINUE READING
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