Gene cloning and characterization of KdeA, a multidrug efflux pump from Klebsiella pneumoniae.

@article{Ping2007GeneCA,
  title={Gene cloning and characterization of KdeA, a multidrug efflux pump from Klebsiella pneumoniae.},
  author={Yu Ping and Wakano Ogawa and Teruo Kuroda and Tomofusa Tsuchiya},
  journal={Biological & pharmaceutical bulletin},
  year={2007},
  volume={30 10},
  pages={1962-4}
}
We cloned a gene related to multidrug resistance from a drug-resistant Klebsiella pneumoniae strain MGH78578. We designated the gene kdeA, which encodes a protein possessing 12 hydrophobic regions. The deduced amino acid sequence of KdeA is similar to that of MdfA, a well-characterized multidrug efflux pump from Escherichia coli. Introduction of the kdeA gene into cells of the drug-hypersusceptible E. coli strain KAM32 resulted in elevated minimum inhibitory concentrations of chloramphenicol… CONTINUE READING

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