Gene cloning and cellular localization of a membrane-bound acid phosphatase of Leishmania mexicana.

@article{Wiese1996GeneCA,
  title={Gene cloning and cellular localization of a membrane-bound acid phosphatase of Leishmania mexicana.},
  author={Martin Wiese and otto. berger and York Stierhof and M Wolfram and Margit Fuchs and Peter Overath},
  journal={Molecular and biochemical parasitology},
  year={1996},
  volume={82 2},
  pages={153-65}
}
In a previous publication, we described the purification of a membrane-bound acid phosphatase of Leishmania mexicana as a heterogeneously N-glycosylated protein of an apparent molecular mass of 70000-72000 expressed in both the promastigote and the amastigote stage of the parasite [19]. Screening of a genomic DNA library of L. mexicana with degenerate oligonucleotides designed according to the NH2-terminus of the protein led to the cloning of the lmmbap gene, which is present in one copy per… CONTINUE READING

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