Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9

@article{Nagata1995GeneCP,
  title={Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9},
  author={Shinji Nagata and S. Bakthavatsalam and Andrey G Galkin and Hironori Asada and Shingo Sakai and Nobuyoshi Esaki and Kenji Soda and Toshihisa Ohshima and Susumu Nagasaki and Haruo Misono},
  journal={Applied Microbiology and Biotechnology},
  year={1995},
  volume={44},
  pages={432-438}
}
A halophilic and thermophilic isolate from the sand of Tottori Dune was found to produce a thermostable and halophilic leucine dehydrogenase (EC 1.4.1.9). It was identified to be a new strain of Bacillus licheniformis. The enzyme gene was cloned into Escherichia coli JM109 with a vector plasmid pUC18. The enzyme was purified to homogeneity from the clone cell extract by ion-exchange column chromatography with a yield of 31%. The enzyme was found to be composed of eight subunits identical in… CONTINUE READING