Gene cloning, expression and characterization of a neoagarotetraose-producing β-agarase from the marine bacterium Agarivorans sp. HZ105.

@article{Lin2012GeneCE,
  title={Gene cloning, expression and characterization of a neoagarotetraose-producing β-agarase from the marine bacterium Agarivorans sp. HZ105.},
  author={Bokun Lin and Guoyong Lu and Yandan Zheng and Wei Xie and Shengkang Li and Zhong Hu},
  journal={World journal of microbiology & biotechnology},
  year={2012},
  volume={28 4},
  pages={1691-7}
}
A β-agarase gene hz2 with 2,868 bp was cloned from the marine agarolytic bacterium Agarivorans sp. HZ105. It encoded a mature agarase HZ2 of 102,393 Da (920 amino acids). Based on the amino acid sequence similarity, agarase HZ2 was assigned to the glycoside hydrolase family 50. The β-agarase shared a gene sequence identity of 98.6% with the reported but much less characterized β-agarase agaB from Vibrio sp. JT0107. Its recombinant agarase rHZ2 was produced in E. coli cells and purified to… CONTINUE READING
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