Gene cloning, expression and characterisation of a new β-agarase, AgWH50C, producing neoagarobiose from Agarivorans gilvus WH0801.

@article{Liu2014GeneCE,
  title={Gene cloning, expression and characterisation of a new β-agarase, AgWH50C, producing neoagarobiose from Agarivorans gilvus WH0801.},
  author={Nan Liu and Xiangzhao Mao and Meng Qin Yang and Bozhong Mu and Dongzhi Wei},
  journal={World journal of microbiology & biotechnology},
  year={2014},
  volume={30 6},
  pages={
          1691-8
        }
}
agWH50C, a novel β-agarase gene, was cloned from Agarivorans gilvus WH0801 by degenerate PCR and nested PCR. The gene agWH50C comprized a 2,223-bp, encoding a protein of 740 amino acids. Sequencing results demonstrated that AgWH50C shared 45 % sequence identity with a well characterized β-agarase, Aga50D, from Saccharophagus degradans 2-40. The mature agarase was expressed in Escherichia coli and purified by affinity chromatography. The optimum pH and temperature for AgWH50C activity were 6.0… CONTINUE READING
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