Gene cloning, expression, and characterization of phenolic acid decarboxylase from Lactobacillus brevis RM84

@article{Landete2010GeneCE,
  title={Gene cloning, expression, and characterization of phenolic acid decarboxylase from Lactobacillus brevis RM84},
  author={Jos{\'e} Mar{\'i}a Landete and H{\'e}ctor Rodr{\'i}guez and Jos{\'e} Antonio Curiel and Blanca de las Rivas and Jos{\'e} Miguel Manche{\~n}o and Rosario Palacios Mu{\~n}oz},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2010},
  volume={37},
  pages={617-624}
}
Phenolic acid decarboxylase (PAD) catalyzes the synthesis of vinyl phenols from hydroxycinnamic acids. The gene encoding PAD from Lactobacillus brevis was cloned and expressed as a fusion protein in Escherichia coli. The recombinant PAD enzyme is a heat-labile enzyme that functions optimally at 22°C and pH 6.0. The purified enzyme did not show thermostability at temperatures above 22°C. L. brevis PAD is able to decarboxylate exclusively the hydroxycinnamic acids, such as p-coumaric, caffeic… CONTINUE READING