Gene amplification mechanisms: the role of fragile sites.

  title={Gene amplification mechanisms: the role of fragile sites.},
  author={Michelle Debatisse and Arnaud Coquelle and Franck Toledo and G{\'e}rard Buttin},
  journal={Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer},
We studied the early stages of gene amplification in a Chinese hamster cell line and identified two distinct amplification mechanisms, both relying on an unequal segregation of gene copies at mitosis. In some cases, a sequence containing the selected gene is looped out, generating an acentric circular molecule, and amplification proceeds through unequal segregation of such extrachromosomal elements in successive cell cycles. In other cases, the accumulation of intrachromosomally amplified… CONTINUE READING