Gene amplification in Rhizobium: identification and in vivo cloning of discrete amplifiable DNA regions (amplicons) from Rhizobium leguminosarum biovar phaseoli.

@article{Flores1993GeneAI,
  title={Gene amplification in Rhizobium: identification and in vivo cloning of discrete amplifiable DNA regions (amplicons) from Rhizobium leguminosarum biovar phaseoli.},
  author={Marlon de Moraes Flores and Susana Brom and Tomasz Maciej Stępkowski and Marianne Girard and Galicia Davila and D. Romero and Rafael Palacios},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 11},
  pages={4932-6}
}
A genetic element that allows the positive selection of different genomic rearrangements was used to analyze DNA amplification in Rhizobium leguminosarum biovar phaseoli. Discrete amplifiable DNA regions (amplicons) were detected in different regions of the genome of the model strain CFN42, including the chromosome and several large plasmids. Amplicons were mobilized into Escherichia coli using a genetic approach that involves the introduction of an origin of replication active in E. coli and… CONTINUE READING

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