Gene affecting longevity of messenger RNA: A mutant ofEscherichia coli with altered mRNA stability

@article{Kuwano1977GeneAL,
  title={Gene affecting longevity of messenger RNA: A mutant ofEscherichia coli with altered mRNA stability},
  author={Michihiko Kuwano and Mayumi Ono and Hideya Endo and Katsuji Hori and Kunihiko Nakamura and Yukinori Hirota and Yoshinari Ohnishi},
  journal={Molecular and General Genetics MGG},
  year={1977},
  volume={154},
  pages={279-285}
}
we have screened 897 temperature sensitive growth mutants ofE. coli for mutant strains showing longer mRNA half-life. The fate of pulse-labelled RNA was examined at 42° C after cessation of RNA synthesis and with prior exposure to nonpermissive temperature (42° C). Eight stains showed altered turn-over of RNA (presumably mRNA), and further analysis on mutant strain JE15144 indicated that the stability of pulse-labeled RNA as well as of tryptophan (trp) mRNA increased four to seven fold over its… CONTINUE READING

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