Gene activation by copy transposition in mating-type switching of a homothallic fission yeast

@article{Egel2004GeneAB,
  title={Gene activation by copy transposition in mating-type switching of a homothallic fission yeast},
  author={Richard Egel and Herbert Gutz},
  journal={Current Genetics},
  year={2004},
  volume={3},
  pages={5-12}
}
SummaryMating-type switching in homothallic clones of the fission yeast, Schizosaccharomyces pombe, appears to follow the same route as previously found for “mutations” from homothallism to heterothallic ⊕ strains. A copy of mat2-P is transposed to and inserted at mat1, where it functionally replaces the mat1-M allele, and only the mat1 segment is expressed (!) to determine the actual mating type: mat1-M(!) mat2-P = ⊖ ⇌ ⊕ = mat1-P(!) mat2-P. This phenomenon has hitherto been concealed by the… 
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In strains that switch using two different mutant P cassettes introduced at the donor loci, it is shown that in such strains wild-type P-cassette DNA is efficiently generated at mat1 through heteroduplex DNA formation and repair.
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TLDR
Mating-type switching in fission yeast provides the opportunity to study how the HR enzymes participate in replication fork restart at single or repeated chromosomal sequences.
A Unique DNA Recombination Mechanism of the Mating/Cell-type Switching of Fission Yeasts: a Review.
TLDR
Recent exciting developments in the understanding of mating-type switching in fission yeasts are summarized and observations are extended to suggest how such a DNA strand-based epigenetic mechanism of cellular differentiation could also operate in diploid organisms.
Recombination and mating-type switching in a ligase-defective mutant of Schizosaccharomyces pombe
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Hyperspeckled mutants of Schizosaccharomyces pombe: frequent mating-type switching without detectable double-strand breaks
TLDR
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Molecular and Cellular Dissection of Mating-Type Switching Steps in Schizosaccharomyces pombe
ABSTRACT A strand-specific imprint (break) controls mating-type switching in fission yeast. By introducing a thiamine repressible promoter upstream of the mat1 locus, we can force transcription
Two tightly linked silent cassettes in the mating-type region of Schizosaccharomyces pombe
  • R. Egel
  • Biology
    Current Genetics
  • 2004
SummaryGenetic evidence is presented for the presence of two silent cassettes mat2-P and mat3- M, which both map to the right of the expressible site mat1 of the mating-type region in
Mating-Type Cassettes: Structure, Switching and Silencing
TLDR
The interplay of both epigenetic events provides a striking example of dynamic chromatin choreography allowing progression into the S. pombe developmental program.
The pedigree pattern of mating-type switching in Schizosaccharomyces pombe
  • R. Egel
  • Biology
    Current Genetics
  • 2004
TLDR
“Miyata's rule”, the previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele.
Cell lineage asymmetry in Schizosaccharomyces pombe: unilateral transmission of a high-frequency state for mating-type switching in diploid pedigrees
TLDR
Sporulation was followed in diploid pedigrees of the fission yeast Schizosacharomyces pombe of the genotype h90//h−U and long series of switch after switch in the same direction (“recurrent swtiching”) were observed in the pedigree.
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