Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction.

Abstract

Recombinant lentiviral vectors are powerful tools to stably manipulate human pluripotent stem cells. They can be used to deliver ectopic genes, shRNAs, miRNAs, or any possible genetic DNA sequence into diving and nondividing cells. Here we describe a general protocol for the production of self-inactivating lentiviral vector particles and their purification… (More)
DOI: 10.1007/7651_2015_221

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