Gene Expression Differences among Three Neurospora Species Reveal Genes Required for Sexual Reproduction in Neurospora crassa

@article{Lehr2014GeneED,
  title={Gene Expression Differences among Three Neurospora Species Reveal Genes Required for Sexual Reproduction in Neurospora crassa},
  author={Nina A. Lehr and Z. Wang and Ning Li and D. A. Hewitt and F. L{\'o}pez-Gir{\'a}ldez and F. Trail and J. Townsend},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
Many fungi form complex three-dimensional fruiting bodies, within which the meiotic machinery for sexual spore production has been considered to be largely conserved over evolutionary time. Indeed, much of what we know about meiosis in plant and animal taxa has been deeply informed by studies of meiosis in Saccharomyces and Neurospora. Nevertheless, the genetic basis of fruiting body development and its regulation in relation to meiosis in fungi is barely known, even within the best studied… Expand
Comparative Genomics and Transcriptomics To Analyze Fruiting Body Development in Filamentous Ascomycetes
TLDR
Comparative transcriptomics indicated that gene expression patterns in developing fruiting bodies of the three species are more similar to each other than to nonsexual hyphae of the same species. Expand
The ancestral levels of transcription and the evolution of sexual phenotypes in filamentous fungi
TLDR
This work examined differences in fruiting body morphology of five filamentous fungi in the Sordariomycetes, culturing them in a common garden environment and profiling genome-wide gene expression at five developmental stages, and reconstructed ancestral gene expression phenotypes, identifying genes with the largest evolved increases across development. Expand
Integrative Activity of Mating Loci, Environmentally Responsive Genes, and Secondary Metabolism Pathways during Sexual Development of Chaetomium globosum
TLDR
Bayesian networks indicated that gene interactions during sexual development have diverged in concert with the capacities both to reproduce asexually and to live a self-compatible versus self-incompatible life cycle, shifting the hierarchical roles of genes associated with conidiation and heterokaryon incompatibility in N. crassa and C. globosum. Expand
Functional Analysis of Developmentally Regulated Genes chs7 and sec22 in the Ascomycete Sordaria macrospora
TLDR
The authors' data indicate that chs7 is dispensable for fruiting body formation in S. macrospora, whereas sec22 is required for ascospore maturation and germination and thus involved in late stages of sexual development. Expand
The Fast-Evolving phy-2 Gene Modulates Sexual Development in Response to Light in the Model Fungus Neurospora crassa
TLDR
Investigation of the evolution of red light sensors in ascomycetes and confirmed—using the model fungus Neurospora crassa—their roles in modulating the asexual-sexual reproduction balance in fungi, suggesting that further study of the function of phytochromes in fungi is critical to reveal the genetic basis of the a sexual-sexual switch responsible for fungal growth and distribution. Expand
Using evolutionary genomics, transcriptomics, and systems biology to reveal gene networks underlying fungal development
TLDR
Recent advances in genome-wide analysis performed in representative multicellular fungal models and non-models have greatly improved upon the traditional reductive approaches in fungal evo-devo research by providing clues for focused knockout strategies. Expand
Is Genome Complexity a Consequence of Inefficient Selection? Evidence from Intron Creation in Nonrecombining Regions.
  • S. Roy
  • Biology, Medicine
  • Molecular biology and evolution
  • 2016
TLDR
Evolution of intron-exon structures in genomic regions of recombination suppression (RRSs), which experience drastically reduced selective efficiency due to hitchhiking and background selection, are studied to suggest that efficiency of selection may not be a major cause of differences in intronic structures across organisms. Expand
New insights from an old mutant: SPADIX4 governs fruiting body development but not hyphal fusion in Sordaria macrospora
TLDR
The results point to SPD4 as a specific determinant of fruiting body formation and research on SPD4 will contribute to understanding cellular reprogramming during initiation of sexual development in fungi. Expand
The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa
TLDR
In both conidiation and the sexual development process,ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. Expand
A Tool Set for the Genome-Wide Analysis of Neurospora crassa by RT-PCR
TLDR
This work has identified genes that are optimal for use as reference genes in RT-PCR across a wide range of expression levels; the mRNA/transcripts from these genes have potential for Use as reference noncycling transcripts outside of Neurospora. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 71 REFERENCES
Global Gene Expression and Focused Knockout Analysis Reveals Genes Associated with Fungal Fruiting Body Development in Neurospora crassa
TLDR
It was found that functionally unclassified proteins accounted for most upregulated genes, whereas downregulated genes were enriched for diverse functions, and genes associated with developmental traits exhibited stage-specific peaks of expression. Expand
The genome sequence of the filamentous fungus Neurospora crassa
TLDR
A high-quality draft sequence of the N. crassa genome is reported, suggesting that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes. Expand
Sex-specific gene expression during asexual development of Neurospora crassa.
TLDR
Significant differences in overall gene expression between mating types across clonal development, especially at late development stages are observed, and significant up-regulation of expression was observed for many late light-responsive genes at late asexual development stages. Expand
Mating type in filamentous fungi.
TLDR
This review focuses on recent molecular analyses of well-characterized mating systems from representative ascomycete and basidiomycete fungi, which involve novel one-to-many specificity in both pheromone-receptor and homeodomain protein interactions. Expand
Mating types and sexual development in filamentous ascomycetes.
TLDR
Diversity of mating systems, still more apparent if the yeasts and the basidiomycetes are taken into account, clearly shows that no single species can serve as a universal mating-type model. Expand
Mating Type and the Genetic Basis of Self-Fertility in the Model Fungus Aspergillus nidulans
TLDR
Results indicate that selfing in A. nidulans involves activation of the same mating pathways characteristic of sex in outcrossing species, i.e., self-fertilization does not bypass requirements for outcrossed sex but instead requires activation of these pathways within a single individual. Expand
A comprehensive phylogeny of Neurospora reveals a link between reproductive mode and molecular evolution in fungi.
TLDR
It is argued that the ancestor of Neurospora was likely heterothallic and that homothallism has evolved independently at least six times in the evolutionary history of the genus, and likelihood ratio tests of substitution rates indicate that reproductive mode is an important factor driving genome evolution in neurospora. Expand
Conflict between reproductive gene trees and species phylogeny among heterothallic and pseudohomothallic members of the filamentous ascomycete genus Neurospora.
TLDR
This study investigated the genealogies of genes important for sexual identity, i.e. mating-type (mat) and pheromone-receptor (pre) genes, among heterothallic and peudohomothallic taxa of Neurospora to argue that reproductive genes are more permeable to gene flow, than other parts of the genome. Expand
Consequences of reproductive mode on genome evolution in fungi.
TLDR
The available data from fungi suggest that reproductive mode alters the rates and patterns of genome evolution in these organisms, e.g., protein evolution, mutation rate, codon usage, frequency of genome rearrangements and repetitive elements, and variation in chromosome size. Expand
Sex-linked transcriptional divergence in the hermaphrodite fungus Neurospora tetrasperma
TLDR
The data show that the genes exhibiting mat-linked expression are enriched in the region genetically linked to mating type, and sequence and expression divergence are positively correlated, which indicates that the phenotype of mat A strains is optimized for traits promoting sexual/female development and the physique of mat a strains for vegetative/male development. Expand
...
1
2
3
4
5
...