published in two parts in 1994 and 1997. The author sig nificantly rearranged the text (in accordance with recent achievement in the filed) and introduced color illustra tions. The monograph by S. N. Shchelkunov significantly differs from many handbooks on methods of gene engi neering. In contrast to well known handbook by Maniatis, Frig, and Sambrook (Methods of Gene Engineering (Molecular Cloning), Mir, Moscow, 1984) this monograph does not contain detailed protocols of meth ods. The author gives comparative description of various approaches for solution of practical problems on DNA cloning. Each researcher can choose an optimal variant. With exception of the section “Methods of chemical enzymatic synthesis of double stranded DNA fragments” written by the distinguished expert in chemical synthesis of oligonucleotides A. N. Sinyakov, the book has been written by one author in one style. This is undoubtedly an advantage of this book compared with the two volume handbook DNA Cloning and New in DNA Cloning (Davis, ed.) translated and published by Mir (Moscow) in 1988. (It should also be noted that both these translations are out of date now). A lecture course on gene engineering given by S. N. Shchelkunov at Novosibirsk University for more than a quarter of a century represents the basis of this mono graph, which consists of 19 chapters. The author sequen tially considers various host–vector systems for Escherichia coli, bacteria of the genera Bacillus, Streptococcus, corinebacteria, yeast Saccharomyces cere visiae, mammalian, and insect cells. Vector systems based on animal viruses are cataloged in detail. Separate chap ters consider site directed mutagenesis and protein engi neering, antiviral vaccines, and transgenic plants and ani mals. Each chapter contains a list of references for further reading, which makes this monograph a valuable refer ence book. The monograph should become a textbook for all specialists working in gene engineering. It is a very impor tant book for subsequent development of biotechnology in our country and it is really a pity that the second edi tion was issued in only 2000 copies. Biochemistry (Moscow), Vol. 70, No. 7, 2005, p. 833. Translated from Biokhimiya, Vol. 70, No. 7, 2005, p. 1008. Original Russian Text Copyright © 2005 by Matvienko, Ovchinnikov.