Gene Deletion by Fluorescence-Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis

@inproceedings{Mueller2016GeneDB,
  title={Gene Deletion by Fluorescence-Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis},
  author={Konrad E. Mueller and Katerina Wolf and Kenneth A. Fields},
  booktitle={mBio},
  year={2016}
}
UNLABELLED Although progress in Chlamydia genetics has been rapid, genomic modification has previously been limited to point mutations and group II intron insertions which truncate protein products. The bacterium has thus far been intractable to gene deletion or more-complex genomic integrations such as allelic exchange. Herein, we present a novel suicide vector dependent on inducible expression of a chlamydial gene that renders Chlamydia trachomatis fully genetically tractable and permits… CONTINUE READING
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Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector

  • L Song, JH Carlson, +9 authors HD Caldwell
  • Appl Environ Microbiol
  • 2013

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