Gene Cluster for Assembly of Pilus Colonization Factor Antigen III of Enterotoxigenic Escherichia coli

@article{Taniguchi2001GeneCF,
  title={Gene Cluster for Assembly of Pilus Colonization Factor Antigen III of Enterotoxigenic Escherichia coli},
  author={T. Taniguchi and Y. Akeda and Ayako Haba and Y. Yasuda and Koichiro Yamamoto and T. Honda and K. Tochikubo},
  journal={Infection and Immunity},
  year={2001},
  volume={69},
  pages={5864 - 5873}
}
ABSTRACT The assembly of pilus colonization factor antigen III (CFA/III) of enterotoxigenic Escherichia coli (ETEC) requires the processing of CFA/III major pilin (CofA) by a prepilin peptidase (CofP), similar to other type IV pilus formation systems. CofA is produced initially as a 26.5-kDa preform pilin (prepilin) and then processed to a 20.5-kDa mature pilin by CofP which is predicted to be localized in the inner membrane. In the present experiment, we determined the nucleotide sequence of… Expand
Structure and secretion of CofJ, a putative colonization factor of enterotoxigenic Escherichia coli
TLDR
A 2.6 Å resolution crystal structure of CofJ is presented, revealing a large β‐sandwich protein that bears no sequence or structural homology to TcpF, suggesting a role in membrane attachment during ETEC colonization. Expand
Structural Characterization of CFA/III and Longus Type IVb Pili from Enterotoxigenic Escherichia coli
TLDR
A direct correlation between CFA/III expression and ETEC aggregation is shown, suggesting that these pili, like the Vibrio cholerae toxin-coregulated pili (TCP), mediate microcolony formation. Expand
Crystal Structure of the Minor Pilin CofB, the Initiator of CFA/III Pilus Assembly in Enterotoxigenic Escherichia coli*
TLDR
A model for CofB-initiated pilus assembly is proposed with implications for understanding filament growth in more complex Type IV pilus systems as well as the related Type II secretion system. Expand
BfpU, a soluble protein essential for type IV pilus biogenesis in enteropathogenic Escherichia coli.
TLDR
Fractionation studies revealed that BfpU represents a novel protein required for type IV pilus assembly that is completely soluble and is detected in both the periplasm and the cytoplasm. Expand
Interplay of a secreted protein with type IVb pilus for efficient enterotoxigenic Escherichia coli colonization
TLDR
The type IVb minor pilin CofB of CFA/III from enterotoxigenic Escherichia coli (ETEC) plays a role not only in T4bP assembly by forming a trimeric initiator complex, but also in bacterial adhesion by anchoring a secreted protein, CofJ, at the trimerization interface of H-type lectin domain. Expand
The Longus type IV pilus of enterotoxigenic Escherichia coli (ETEC) mediates bacterial self-aggregation and protection from antimicrobial agents.
TLDR
Longus-expressing ETEC strains were associated with improved survival when exposed to antibacterial factors including lysozyme and antibiotics, which suggests that longus-mediated bacterial self-aggregates protect bacteria against antimicrobial environmental agents and may promote gut colonization. Expand
Homo-trimeric Structure of the Type IVb Minor Pilin CofB Suggests Mechanism of CFA/III Pilus Assembly in Human Enterotoxigenic Escherichia coli.
In gram-negative bacteria, the assembly of type IV pilus (T4P) and the evolutionally related pseudopilus of type II secretion system involves specialized structural proteins called pilins andExpand
Identification of a novel type IV pilus gene cluster required for gastrointestinal colonization of Citrobacter rodentium
TLDR
Citrobacter rodentium provides a convenient model to study type IV pilus‐mediated pathogen–host interactions under physiological conditions in the natural colonic environment. Expand
Cloning, expression, purification, crystallization and X-ray crystallographic analysis of CofB, the minor pilin subunit of CFA/III from human enterotoxigenic Escherichia coli.
  • K. Kawahara, H. Oki, +8 authors T. Ohkubo
  • Chemistry, Medicine
  • Acta crystallographica. Section F, Structural biology communications
  • 2015
TLDR
The initial phase was solved by the single-wavelength anomalous dispersion (SAD) method using a dehydrated SeMet CofB crystal, which resulted in an interpretable electron-density map. Expand
Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 Å resolution by sulfur-SAD phasing.
TLDR
The CofA structure showed the αβ-fold typical of type IVb pilins and showed high structural homology to that of TcpA for toxin-coregulated pili of Vibrio cholerae, including spatial distribution of key residues critical for pilin self-assembly. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 55 REFERENCES
The Gene Encoding the Prepilin Peptidase Involved in Biosynthesis of Pilus Colonization Factor Antigen III (CFA/III) of Human Enterotoxigenic Escherichia coli
TLDR
The structural gene, cofP, encoding CFA/III prepilin peptidase which cleavages at the Gly‐30‐Met‐31 junction of CofA was identified, and the nucleotide sequence of the gene was determined. Expand
Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili
TLDR
Results suggest that the major pilin of CFA/III pili is produced as a precursor form which is posttranslationally modified to the mature pilin and forms morphological pili after cleavage of the Gly-30-Met-31 junction, probably by a protease encoded by an as-yet-unknown gene located downstream of cofA. Expand
Longus pilus of enterotoxigenic Escherichia coli and its relatedness to other type-4 pili--a minireview.
Longus is a long pilus produced by human enterotoxigenic Escherichia coli (ETEC) which shares significant structural and biochemical features with class-B type-4 pili. These pili include theExpand
Identification of IngA, the structural gene of longus type IV pilus of enterotoxigenic Escherichia coli
TLDR
Transmission electron microscopy and immunoblot analysis of ETEC strains expressing either longus or CFA/III detected antigenic differences between native and denatured epitopes of these pili. Expand
Identification of lngA, the structural gene of longus type IV pilus of enterotoxigenic Escherichia coli.
TLDR
Transmission electron microscopy and immunoblot analysis of ETEC strains expressing either longus or CFA/III detected antigenic differences between native and denatured epitopes of these pili. Expand
Enteropathogenic Escherichia coli: identification of a gene cluster coding for bundle-forming pilus morphogenesis
TLDR
Restriction fragment length polymorphism analysis of DNA from six additional EPEC serotypes showed that the organization of the bfp gene cluster and its juxtaposition with a potential plasmid origin of replication are highly conserved features of the EPEC biotype. Expand
A cluster of fourteen genes from enteropathogenic Escherichia coli is sufficient for the biogenesis of a type IV pilus
TLDR
It is shown that expression of the 14 genes in the bfp cluster from an IPTG‐inducible promoter, in the absence of pJPN14, is sufficient to reconstitute BFP biogenesis in a laboratory E. coli strain with a defined set of genes. Expand
New tools in an old trade: CS1 pilus morphogenesis
TLDR
The proteins of CS1 pili have no significant homology to those of the well‐characterized Pap (pyelonephritis‐associated) pili and related systems, although most of the features of pilus morphogenesis are similar, and appear to be among the rare cases of convergent evolution. Expand
Genes for biosynthesis and assembly of CS3 pili of CFA/II enterotoxigenic Escherichia coli: novel regulation of pilus production by bypassing an amber codon
TLDR
The complete nucleotide sequence of the 4746bp HindIII fragment encoding the genes for the biosynthesis and assembly of CS3 pili has been determined and the actual reading frames for the various products have been determined. Expand
BfpB, an outer membrane lipoprotein required for the biogenesis of bundle-forming pili in enteropathogenic Escherichia coli
TLDR
Biochemical and cell fractionation studies showed that BFPB is a 58-kDa lipoprotein that is located primarily in the outer membrane, indicating that BfpB acts at a step in the BFP biogenic pathway after production and processing of the structural subunit. Expand
...
1
2
3
4
5
...