Gene Amplification Can Correct a Photosynthetic Growth Defect Caused by mRNA Instability in Chlamydomonas Chloroplasts.

@article{Kindle1994GeneAC,
  title={Gene Amplification Can Correct a Photosynthetic Growth Defect Caused by mRNA Instability in Chlamydomonas Chloroplasts.},
  author={Karen L. Kindle and Hideho Suzuki and David B Stern},
  journal={The Plant cell},
  year={1994},
  volume={6 2},
  pages={
          187-200
        }
}
Chlamydomonas reinhardtii chloroplast transformants that lack an inverted repeat normally found at the 3[prime] end of the chloroplast atpB gene have a slow phototrophic growth phenotype due to reduced accumulation of atpB mRNA and the chloroplast ATPase [beta] subunit. We have recovered transformants exhibiting more robust phototrophic growth at a moderate frequency (~1% relative to slow-growing transformants). Quantitative DNA blot analysis indicated that in one class of these robust… CONTINUE READING

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