GenIUL , experts on viability PCR

  title={GenIUL , experts on viability PCR},
  author={Gemma Agust{\'i} and Francesc Codony},
The standard PCR technique allows the rapid detection of microorganisms, difficult to detect by traditional culture methods. However, the control of samples through PCR has the drawback of false positives due to amplification of DNA/RNA of non-viable cells. This can be overcome by adding intercalating agents (EMA or PMA) to the sample, which selectively penetrates nonviable cells and blocks their DNA/RNA after a photo-activation process, preventing its subsequent amplification by PCR. This… CONTINUE READING