Gel electrophoresis of giant proteins: solubilization and silver-staining of titin and nebulin from single muscle fiber segments.

@article{Granzier1993GelEO,
  title={Gel electrophoresis of giant proteins: solubilization and silver-staining of titin and nebulin from single muscle fiber segments.},
  author={Henk L. M. Granzier and Kuan Wang},
  journal={Electrophoresis},
  year={1993},
  volume={14 1-2},
  pages={56-64}
}
Giant proteins in the megadalton range (> 0.5 MDa) appear to play important structural and functional roles in striated muscle. Titin (approximately 3 MDa) is involved in the generation of resting tension and the assembly and stability of the sarcomere in skeletal and cardiac muscle tissues, while nebulin (approximately 0.7 MDa) is thought to regulate thin filament length in skeletal muscle. Sodium dodecyl sulfate (SDS)-gel electrophoresis is an important tool in revealing the size, quantity… CONTINUE READING