Gel diffusion assays for endo-beta-mannanase and pectin methylesterase can underestimate enzyme activity due to proteolytic degradation: a remedy.

@article{Bourgault2002GelDA,
  title={Gel diffusion assays for endo-beta-mannanase and pectin methylesterase can underestimate enzyme activity due to proteolytic degradation: a remedy.},
  author={Richard Bourgault and J. Derek Bewley},
  journal={Analytical biochemistry},
  year={2002},
  volume={300 1},
  pages={87-93}
}
The accuracy of the sensitive gel-diffusion assay for endo-beta-mannanase activity was improved when protein was added to fruit extracts or into the substrate-gel matrix in which the enzyme assays were conducted. Mixing of commercially available protease inhibitors with fruit enzyme extracts also resulted in increased assayable activity. These treatments… CONTINUE READING