Gel-based fluorescence resonance energy transfer (gelFRET) analysis of nucleoprotein complex architecture.

@article{RamirezCarrozzi2001GelbasedFR,
  title={Gel-based fluorescence resonance energy transfer (gelFRET) analysis of nucleoprotein complex architecture.},
  author={Vladimir R. Ramirez-Carrozzi and Tom Klaus Kerppola},
  journal={Methods},
  year={2001},
  volume={25 1},
  pages={31-43}
}
A gel-based fluorescence resonance energy transfer (gelFRET) assay was developed for analysis of the architecture of nucleoprotein complexes. gelFRET is based on fluorescence analysis of nucleoprotein complexes separated by polyacrylamide gel electrophoresis. These complexes are separated from free components and nonspecific complexes, enabling fluorescence analysis of complexes containing all components in stoichiometric proportions. gelFRET can be used to investigate the structural… CONTINUE READING

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