Gel-absorption-based sample preparation method for shotgun analysis of membrane proteome.


Membrane proteins solubilized in a starting buffer containing high concentration of sodium dodecyl sulfate (SDS) are directly entrapped and immobilized into gel matrix when the membrane protein solution is absorbed by the vacuum-dried polyacrylamide gel. After the detergent and other salts are removed by washing, the proteins are subjected to in-gel… (More)
DOI: 10.1007/978-1-61779-821-4_31


Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.