Gel Electrophoresis - Principles and Basics

@inproceedings{Magdeldin2012GelE,
  title={Gel Electrophoresis - Principles and Basics},
  author={Sameh Magdeldin},
  year={2012}
}
An Overview of the Potential Therapeutic Applications of Essential Oils
TLDR
The antimicrobial effects of Eo, modes of EO action (membrane disruption, efflux inhibition, increase membrane permeability, and decrease in intracellular ATP), and their compounds' potential as effective agents against bacteria, fungi, and viruses are reviewed. Expand
Modeling the Impact of Sensitivity Distribution Variations of Tetrapolar Impedance Configurations in Microfluidic Analytical Devices
Goal: The purpose of this work is to investigate the effects of electrode topology and electrode configuration of tetrapole electrical impedance measurement (TEIM) systems used in microfluidicExpand
The Impact of DNA Methylation on IL6 mRNA Levels in Hematinic Deficiency and Atopy-Associated Recurrent Aphthous Stomatitis Patients
TLDR
This study showed the upregulation of IL6 mRNA levels in RAS patients compared to control, and there was a significant relationship between the expression of the IL6 gene and the increased risk of RAS. Expand
Cloning and Expression of Pseudomonas aeruginosa AlkB Gene in E. coli
Pre identified hydrocarbons degrading bacteria were used in this study, specific primer was conducted to amplification of AlkB gene, approximately 1206bp band size of this gene for PseudomonasExpand
DETERMINATION OF DNA QUALITY EXTRACTED FROM FOOD PRODUCTS
Deoxyribonucleic acid or DNA is the hereditary molecule of living things. Food products mostly contain ingredients originated from animals and plants. Food adulteration is the main concern in foodExpand
Estimation of Genetic Diversity in Four Genetic Groups of Local Kurdish Chicken Using RAPD- PCR
Keywards: Kurdish Chicken, Molecular Genetics, Characterization, RAPD. يوونلا ضماحلل يئاوشعلا ميخضتلا ةينقت مادختسأب يلحملا يدروكلا جاجدلا نم ةيثارو تاعومجم عبرا يف يثارولا عونتلا ريدقت لاكشلاا ددعتم
Expression of Recombinant Fusion Protein of Newcastle Disease Virus from Escherichia coli Plasmid Clone C-2a by In-vitro Cell-free Protein Expression System
TLDR
This study confirmed that clone C-2a of E.coli carrying a recombinant plasmid pBT7-N-His-Fusion NDV carried a specific protein fragment with molecular weight of 25,6 kDa which was inserted by recombinant F protein of NDV gene. Expand
Proteomic Studies of Micronutrient Deficiency and Toxicity
TLDR
Proteomic analysis may help in identifying novel protein target that will determine whether these micronutrients do affect other metabolic pathways or not and will provide detailed functional aspects of micronsutrients which will enable us to understand the underlying mechanism properly. Expand
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Potential new inorganic antitumour agents from combining the anticancer traditional Chinese medicine (TCM) matrine with Ga(III), Au(III), Sn(IV) ions, and DNA binding studies.
Three new compounds of Ga(III), Au(III), Sn(IV) with matrine (MT), [H-MT][GaCl(4)] (1), [H-MT][AuCl(4)] (2) and [Sn(H-MT)Cl(5)] (3), have been synthesized and characterized by elemental analysis, IR,Expand
DNA binding affinity and antioxidative activity of copper(II) and zinc(II) complexes with a novel hesperetin Schiff base ligand
A hesperetin Schiff base ligand (H(4)L) and its complexes, [H(3)CuL center dot OAc]center dot H(2)O and [H(3)ZnL center dot OAc]center dot 2H(2)O, have been synthesized and characterized on the basisExpand
Three new mono–di–trinuclear cobalt complexes of selectively and non-selectively condensed Schiff bases with N2O and N2O2 donor sets: Syntheses, structural variations, EPR and DNA binding studies
Abstract Three new mono–di–trinuclear cobalt complexes of three different Schiff bases have been synthesised. In all the three complexes psuedohalides (NCO− and N3−) have been incorporated toExpand
Novel copper(II) complex of N-propyl-norfloxacin and 1,10-phenanthroline with enhanced antileukemic and DNA nuclease activities.
TLDR
It is found that the proliferation rate and viability of HL-60 cells decreased after treatment with complex 1, leading to cell death through apoptosis in a time-dependent manner, suggesting that 1 may provide a valuable tool in cancer chemotherapy. Expand
DNA hydrolytic cleavage by stable Zn(II) dipeptide complexes
Interaction of zinc with dipeptides cysteinylglycine, histidylphenylalanine and histidylserine has been investigated at 35°C and 0.1 M ionic strength and the stabilities of these complexesExpand
History and principles of conductive media for standard DNA electrophoresis.
TLDR
Low-molarity conductive media are developed that can be reengineered for lower ionic strength, higher field strengths, and lower requirements for heat dissipation in DNA electrophoresis applications. Expand
Hydrolytic Cleavage of DNA by Ternary Amino Acid Schiff Base Copper(II) Complexes Having Planar Heterocyclic Ligands
The ternary copper(II) complexes [Cu(salgly)L] (L = phen, 1; dpq, 2), [Cu(salala)L] (L = phen, 3; dpq, 4) and [Cu(salphe)L] (L = phen, 5; dpq, 6), where salgly, salala and salphe are tridentateExpand
DNA hydrolytic cleavage by the diiron(III) complex Fe(2)(DTPB)(mu-O)(mu-Ac)Cl(BF(4))(2): comparison with other binuclear transition metal complexes.
TLDR
It is established that the dominant factors for the DNA hydrolysis activities of the binuclear metal complexes are the mu-oxo bridge, labile and anionic ligands, and open coordination site(s). Expand
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