Gaussian approximations of fluorescence microscope point-spread function models.

Abstract

We comprehensively study the least-squares Gaussian approximations of the diffraction-limited 2D-3D paraxial-nonparaxial point-spread functions (PSFs) of the wide field fluorescence microscope (WFFM), the laser scanning confocal microscope (LSCM), and the disk scanning confocal microscope (DSCM). The PSFs are expressed using the Debye integral. Under an L… (More)

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Cite this paper

@article{Zhang2007GaussianAO, title={Gaussian approximations of fluorescence microscope point-spread function models.}, author={Bo Zhang and Josiane Zerubia and Jean-Christophe Olivo-Marin}, journal={Applied optics}, year={2007}, volume={46 10}, pages={1819-29} }