Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.

@article{Nallamsetty2005GatewayVF,
  title={Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.},
  author={Sreedevi Nallamsetty and Brian P. Austin and Kerri J. Penrose and David S Waugh},
  journal={Protein science : a publication of the Protein Society},
  year={2005},
  volume={14 12},
  pages={2964-71}
}
Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purification. To compensate for this shortcoming, we have engineered and successfully tested Gateway… CONTINUE READING
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A Generic method for the production of recombinant proteins in Escherichia coli using a dual His6-MBP affinity tag

  • J. E. Tropea, S. Cherry, S. Nallamsetty, C. Bignon, D. S. Waugh
  • Methods Mol. Biol. (in press).
  • 2005
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